subcloning and expression of recombinant echinococcus granulosus antigen b, in pqe-30 expression vector

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cultured. the subcloned gene was expressed by iptg. subcloning of gene was confirmed by both pcr and enzyme digestion. results: production of recombinant protein was confirmed by sds-page. western blot analysis was carried out by both his-tag monoclonal ab and human serum to estimate the expressed protein in e. coli cells. recombinant protein was purified and its specificity was proved by western blotting. conclusion: production of this recombinant protein can increase sensitivity and specificity in serological test (elisa).